Polyomavirus origin for DNA replication comprises multiple genetic elements
نویسندگان
چکیده
منابع مشابه
Elements of the polyomavirus replication origin required for homologous recombination mediated by large T antigen.
We introduced various elements of the polyomavirus origin of DNA replication into the genome of rat cells, and we analyzed their capacity to elicit rearrangements within the integrated sequences when exposed to large T antigen. The cis-acting sequences required for homologous recombination were those that make up a functional replication origin.
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A yeast autonomously replicating sequence, ARS305, shares essential components with a chromosome III replicator, ORI305. Known components include an ARS consensus sequence (ACS) element, presumed to bind the origin recognition complex (ORC), and a broad 3'-flanking sequence which contains a DNA unwinding element. Here linker substitution mutagenesis of ARS305 and analysis of plasmid mitotic sta...
متن کاملEffect of silencer on polyomavirus DNA replication.
We have cloned the cellular sequence termed box DNA from the enhancer region of polyomavirus F9 mutant fPyF9. Box DNA functions as a negative transcriptional element (silencer) in undifferentiated F9 cells but not in differentiated L cells. Plasmid DNAs containing the origin and enhancer of polyomavirus were used to measure simultaneously transcriptional and replication activities in transfecte...
متن کاملPolyomavirus large T antigen binds cooperatively to its multiple binding sites in the viral origin of DNA replication.
Polyomavirus large T antigen binds to multiple 5'-G(A/G)GGC-3' pentanucleotide sequences in sites 1/2, A, B, and C within and adjacent to the origin of viral DNA replication on the polyomavirus genome. We asked whether the binding of large T antigen to one of these sites could influence binding to other sites. We discovered that binding to origin DNA is substantially stronger at pH 6 to 7 than ...
متن کاملTwo spatially distinct genetic elements constitute a bipartite DNA replication origin in the minute virus of mice genome.
Mutations were introduced into plasmid pMM984, a full-length infectious clone of the fibrotropic strain of minute virus of mice, to identify cis-acting genetic elements required for the excision and replication of the viral genome. The replicative capacity of these mutants was measured directly, using an in vivo transient DNA replication assay following transfection of plasmids into murine A9 c...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1983
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.47.3.586-599.1983